Journal:

Article Title: Effects of ?-galactosylceramide (KRN7000), interleukin-12 and interleukin-7 on phenotype and cytokine profile of human V?24 + V?11 + T cells

doi: 10.1046/j.1365-2567.1999.00920.x

Figure Lengend Snippet: Phenotype of PB Vα24+ Vβ11+ T cells before and after a 7‐day culture in the presence of KRN7000. PB Vα24+ Vβ11+ T cells (open bars, n = 6) and KRN7000‐cultured Vα24+ Vβ11+ T cells (closed bars, n = 3) were phenotypically analysed using three‐colour flow cytometry. Mean + SD of Vα24+ Vβ11+ T cells positive for various markers is shown. IL‐4 and IFN‐γ expression were assessed before [spontaneous (S)] and after [induced (I)] a 4‐hr stimulation with PMA and calcium‐ionophore.

Article Snippet: KRN7000 ((2 S ,3 S ,4 R )‐1‐ O (α‐ d ‐galactopyranosyl)‐2‐( N ‐hexacosanoylamino)‐1,3‐4‐octadecanetriol) was synthesized by the Pharmaceutical Research Laboratory, Kirin Brewery.

Techniques: Cell Culture, Flow Cytometry, Expressing

Journal:

Article Title: Effects of ?-galactosylceramide (KRN7000), interleukin-12 and interleukin-7 on phenotype and cytokine profile of human V?24 + V?11 + T cells

doi: 10.1046/j.1365-2567.1999.00920.x

Figure Lengend Snippet: KRN7000 induces activation of PB Vα24+ Vβ11+ T cells but not of PB Vα24– Vβ11+ T cells or of PB Vα24+ Vβ11– T cells. PBMC were cultured in the presence of KRN7000 for 7 days. Using three‐colour flow‐cytometry, CD25 expression was analysed in Vα24+ Vβ11+ T cells (a), Vα24– Vβ11+ T cells (b) and Vα24+ Vβ11– T cells (c). Representative dot‐plots of donor 1 are shown.

Article Snippet: KRN7000 ((2 S ,3 S ,4 R )‐1‐ O (α‐ d ‐galactopyranosyl)‐2‐( N ‐hexacosanoylamino)‐1,3‐4‐octadecanetriol) was synthesized by the Pharmaceutical Research Laboratory, Kirin Brewery.

Techniques: Activation Assay, Cell Culture, Flow Cytometry, Expressing

Journal:

Article Title: Effects of ?-galactosylceramide (KRN7000), interleukin-12 and interleukin-7 on phenotype and cytokine profile of human V?24 + V?11 + T cells

doi: 10.1046/j.1365-2567.1999.00920.x

Figure Lengend Snippet: Effects of KRN7000, IL‐12 and IL‐7 on GrB expression in Vα24+ Vβ11+ T cells. GrB expression was assessed in Vα24+ Vβ11+ T cells that were either freshly isolated (pre), cultured for 7 days in the presence of KRN7000 (KRN) or cultured for 7 days in the presence of KRN7000 plus IL‐12 or IL‐7 during the last 24 hr. Data of three donors are shown (donor 1, closed bars; donor 2, open bars; donor 3,hatched bars).

Article Snippet: KRN7000 ((2 S ,3 S ,4 R )‐1‐ O (α‐ d ‐galactopyranosyl)‐2‐( N ‐hexacosanoylamino)‐1,3‐4‐octadecanetriol) was synthesized by the Pharmaceutical Research Laboratory, Kirin Brewery.

Techniques: Expressing, Isolation, Cell Culture

Journal:

Article Title: Effects of ?-galactosylceramide (KRN7000), interleukin-12 and interleukin-7 on phenotype and cytokine profile of human V?24 + V?11 + T cells

doi: 10.1046/j.1365-2567.1999.00920.x

Figure Lengend Snippet: Effects of KRN7000, IL‐12 and IL‐7 on GrB expression by Vα24+ Vβ11+ T cells. GrB expression was analysed using three‐colour flow cytometry. Expression of GrB in PB Vα24+ Vβ11+ T cells that were cultured for 7 days in the presence of KRN7000 is shown (a). Addition of IL‐12 for 24 hr increased GrB levels (b), while addition of IL‐7 decreased GrB levels (c). Representative dot‐plots of donor 2 are shown.

Article Snippet: KRN7000 ((2 S ,3 S ,4 R )‐1‐ O (α‐ d ‐galactopyranosyl)‐2‐( N ‐hexacosanoylamino)‐1,3‐4‐octadecanetriol) was synthesized by the Pharmaceutical Research Laboratory, Kirin Brewery.

Techniques: Expressing, Flow Cytometry, Cell Culture

Journal:

Article Title: Effects of ?-galactosylceramide (KRN7000), interleukin-12 and interleukin-7 on phenotype and cytokine profile of human V?24 + V?11 + T cells

doi: 10.1046/j.1365-2567.1999.00920.x

Figure Lengend Snippet: Effects of KRN7000 and IL‐12 on GrB expression by Vα24+ Vβ11+ T‐cell clones. Clone C14 and allogeneic moDC, matured in the presence of either KRN7000 (bold line) or vehicle (line), were co‐cultured for 48 hr and GrB expression was assessed by flow cytometry, the dotted line represents isotype control (a). IL‐12 induces a time‐dependent up‐regulation of GrB expression in clone B11. Zero hours (solid line), 24 hr (dotted line) and 48 hr (bold line) (b).

Article Snippet: KRN7000 ((2 S ,3 S ,4 R )‐1‐ O (α‐ d ‐galactopyranosyl)‐2‐( N ‐hexacosanoylamino)‐1,3‐4‐octadecanetriol) was synthesized by the Pharmaceutical Research Laboratory, Kirin Brewery.

Techniques: Expressing, Clone Assay, Cell Culture, Flow Cytometry, Control

Journal: Food Chemistry: X

Article Title: Structure-dependent effects of sweet and sweet taste affecting compounds on their sensorial properties

doi: 10.1016/j.fochx.2020.100100

Figure Lengend Snippet: Test compounds and the concentrations (conc.) used [g/L] in the present study, M logP, viscosity and molecular structure. Compounds tested in concentrations exceeding the common or realistic use levels are labelled with #; compounds, which did not reach the required sweetness level of a 5% sucrose solution are labelled with *.

Article Snippet: Acesulfame K, advantame, aspartame, hesperetin sodium salt, iron lactate-II hydrate, maltitol, maltose, neotame, phyllodulcin, phloretin, rebaudioside (reb) A (nat., 99%), reb C, reb D, reb E (contains 20% reb D) and reb M, rubusoside, saccharin sodium salt, sodium cyclamate, sorbitol ( d -), stevioside, sucralose, tannic acid (nat.), thaumatin B (pur) and trehalose were kindly provided in food grade (FG) quality by Symrise AG (Holzminden, Germany).

Techniques: Viscosity

Journal: Journal of Food Science and Technology

Article Title: Development of high protein, high fiber smoothie as a grab-and-go breakfast option using response surface methodology

doi: 10.1007/s13197-017-2841-z

Figure Lengend Snippet: Experimental design run and corresponding responses of breakfast smoothie

Article Snippet: Sucralose and trisodium citrate were purchased from Hi-Media Chemicals, Maharashtra, India, and pectin (high methoxy), from CP Kelco, Division of M/s JM Huber Pvt.

Techniques: Viscosity

Journal: Journal of Food Science and Technology

Article Title: Development of high protein, high fiber smoothie as a grab-and-go breakfast option using response surface methodology

doi: 10.1007/s13197-017-2841-z

Figure Lengend Snippet: Regression coefficients and ANOVA of fitted quadratic model for sensory and physical responses

Article Snippet: Sucralose and trisodium citrate were purchased from Hi-Media Chemicals, Maharashtra, India, and pectin (high methoxy), from CP Kelco, Division of M/s JM Huber Pvt.

Techniques: Viscosity